recombinant proteins advanced dmem f12 gibco Search Results


recombinant proteins advanced dmem f12 gibco  (Thermo Fisher)
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Thermo Fisher recombinant proteins advanced dmem f12 gibco
Recombinant Proteins Advanced Dmem F12 Gibco, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmem f12 culture media  (R&D Systems)
99
R&D Systems dmem f12 culture media
Dmem F12 Culture Media, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmem /nutrient f-12 ham  (Millipore)
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Millipore dmem /nutrient f-12 ham
Dmem /Nutrient F 12 Ham, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant cxcl-10  (R&D Systems)
90
R&D Systems recombinant cxcl-10
Cytokines in cell-free culture supernatants of senescent hepatocytes. (A) Representative images of cytokine arrays. 1, CCL-2; 2, CXCL-1; 3, <t>CXCL-10.</t> (B) Bar graph representing the densitometry of the spotted antibody array results. The secretion of IL-1α, IL-1β, IL-6, CCL-2, CXCL-1 and CXCL-2 was differentially increased. (C) ELISA analysis of CXCL-9, −10 and −11. The levels of only CXCL-10 exhibited significant augmentation. n=5. Data were analyzed by Student's t-test. **P<0.01. IL, interleukin; CCL, C-C motif chemokine ligand; CXCL, C-X-C motif chemokine ligand.
Recombinant Cxcl 10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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npc culturing medium  (Thermo Fisher)
99
Thermo Fisher npc culturing medium
Cytokines in cell-free culture supernatants of senescent hepatocytes. (A) Representative images of cytokine arrays. 1, CCL-2; 2, CXCL-1; 3, <t>CXCL-10.</t> (B) Bar graph representing the densitometry of the spotted antibody array results. The secretion of IL-1α, IL-1β, IL-6, CCL-2, CXCL-1 and CXCL-2 was differentially increased. (C) ELISA analysis of CXCL-9, −10 and −11. The levels of only CXCL-10 exhibited significant augmentation. n=5. Data were analyzed by Student's t-test. **P<0.01. IL, interleukin; CCL, C-C motif chemokine ligand; CXCL, C-X-C motif chemokine ligand.
Npc Culturing Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmem f12  (Thermo Fisher)
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Thermo Fisher dmem f12
Cytokines in cell-free culture supernatants of senescent hepatocytes. (A) Representative images of cytokine arrays. 1, CCL-2; 2, CXCL-1; 3, <t>CXCL-10.</t> (B) Bar graph representing the densitometry of the spotted antibody array results. The secretion of IL-1α, IL-1β, IL-6, CCL-2, CXCL-1 and CXCL-2 was differentially increased. (C) ELISA analysis of CXCL-9, −10 and −11. The levels of only CXCL-10 exhibited significant augmentation. n=5. Data were analyzed by Student's t-test. **P<0.01. IL, interleukin; CCL, C-C motif chemokine ligand; CXCL, C-X-C motif chemokine ligand.
Dmem F12, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmem/f12 medium  (Thermo Fisher)
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Thermo Fisher dmem/f12 medium
Cytokines in cell-free culture supernatants of senescent hepatocytes. (A) Representative images of cytokine arrays. 1, CCL-2; 2, CXCL-1; 3, <t>CXCL-10.</t> (B) Bar graph representing the densitometry of the spotted antibody array results. The secretion of IL-1α, IL-1β, IL-6, CCL-2, CXCL-1 and CXCL-2 was differentially increased. (C) ELISA analysis of CXCL-9, −10 and −11. The levels of only CXCL-10 exhibited significant augmentation. n=5. Data were analyzed by Student's t-test. **P<0.01. IL, interleukin; CCL, C-C motif chemokine ligand; CXCL, C-X-C motif chemokine ligand.
Dmem/F12 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmem/f-12 plus basal medium  (Millipore)
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Millipore dmem/f-12 plus basal medium
Cytokines in cell-free culture supernatants of senescent hepatocytes. (A) Representative images of cytokine arrays. 1, CCL-2; 2, CXCL-1; 3, <t>CXCL-10.</t> (B) Bar graph representing the densitometry of the spotted antibody array results. The secretion of IL-1α, IL-1β, IL-6, CCL-2, CXCL-1 and CXCL-2 was differentially increased. (C) ELISA analysis of CXCL-9, −10 and −11. The levels of only CXCL-10 exhibited significant augmentation. n=5. Data were analyzed by Student's t-test. **P<0.01. IL, interleukin; CCL, C-C motif chemokine ligand; CXCL, C-X-C motif chemokine ligand.
Dmem/F 12 Plus Basal Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biliary single cell media  (Thermo Fisher)
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Thermo Fisher biliary single cell media
Cytokines in cell-free culture supernatants of senescent hepatocytes. (A) Representative images of cytokine arrays. 1, CCL-2; 2, CXCL-1; 3, <t>CXCL-10.</t> (B) Bar graph representing the densitometry of the spotted antibody array results. The secretion of IL-1α, IL-1β, IL-6, CCL-2, CXCL-1 and CXCL-2 was differentially increased. (C) ELISA analysis of CXCL-9, −10 and −11. The levels of only CXCL-10 exhibited significant augmentation. n=5. Data were analyzed by Student's t-test. **P<0.01. IL, interleukin; CCL, C-C motif chemokine ligand; CXCL, C-X-C motif chemokine ligand.
Biliary Single Cell Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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serum-free dmem/f-12 medium supplemented with 20 ng/ml human recombinant epidermal growth factor (egf; millipore, ma, usa)  (Millipore)
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Millipore serum-free dmem/f-12 medium supplemented with 20 ng/ml human recombinant epidermal growth factor (egf; millipore, ma, usa)
CCL18 involves in M2-like TAMs induced EMT and stemness in SCCHN in vitro. Tu686 cells cultured with M2 CM or M0 CM in the presence or absence of an anti-CCL18 neutralizing antibody at 15 μg/mL, or an isotype-matched IgG protein. a – d qRT-PCR assays were used to check mRNA expression of EMT markers E-cadherin ( a ), Vimentin ( b ), and EMT transcription factors Snail ( c ) and Slug ( d ). e , f Representative images for <t>tumorsphere</t> formation and the number of tumorspheres. g , h mRNA expression of CSCs markers CD133 ( g ) and CD44 ( h ) was quantified by qPCR. Data are shown as mean ± SD. * P < 0.05, ** P <0.01, *** P < 0.001, **** P < 0.0001
Serum Free Dmem/F 12 Medium Supplemented With 20 Ng/Ml Human Recombinant Epidermal Growth Factor (Egf; Millipore, Ma, Usa), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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murine recombinant epidermal growth factor peprotech #315-09  (PeproTech)
90
PeproTech murine recombinant epidermal growth factor peprotech #315-09
CCL18 involves in M2-like TAMs induced EMT and stemness in SCCHN in vitro. Tu686 cells cultured with M2 CM or M0 CM in the presence or absence of an anti-CCL18 neutralizing antibody at 15 μg/mL, or an isotype-matched IgG protein. a – d qRT-PCR assays were used to check mRNA expression of EMT markers E-cadherin ( a ), Vimentin ( b ), and EMT transcription factors Snail ( c ) and Slug ( d ). e , f Representative images for <t>tumorsphere</t> formation and the number of tumorspheres. g , h mRNA expression of CSCs markers CD133 ( g ) and CD44 ( h ) was quantified by qPCR. Data are shown as mean ± SD. * P < 0.05, ** P <0.01, *** P < 0.001, **** P < 0.0001
Murine Recombinant Epidermal Growth Factor Peprotech #315 09, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fetal bovine serum fbs 1x phosphate buffered saline  (Thermo Fisher)
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Thermo Fisher fetal bovine serum fbs 1x phosphate buffered saline
CCL18 involves in M2-like TAMs induced EMT and stemness in SCCHN in vitro. Tu686 cells cultured with M2 CM or M0 CM in the presence or absence of an anti-CCL18 neutralizing antibody at 15 μg/mL, or an isotype-matched IgG protein. a – d qRT-PCR assays were used to check mRNA expression of EMT markers E-cadherin ( a ), Vimentin ( b ), and EMT transcription factors Snail ( c ) and Slug ( d ). e , f Representative images for <t>tumorsphere</t> formation and the number of tumorspheres. g , h mRNA expression of CSCs markers CD133 ( g ) and CD44 ( h ) was quantified by qPCR. Data are shown as mean ± SD. * P < 0.05, ** P <0.01, *** P < 0.001, **** P < 0.0001
Fetal Bovine Serum Fbs 1x Phosphate Buffered Saline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Cytokines in cell-free culture supernatants of senescent hepatocytes. (A) Representative images of cytokine arrays. 1, CCL-2; 2, CXCL-1; 3, CXCL-10. (B) Bar graph representing the densitometry of the spotted antibody array results. The secretion of IL-1α, IL-1β, IL-6, CCL-2, CXCL-1 and CXCL-2 was differentially increased. (C) ELISA analysis of CXCL-9, −10 and −11. The levels of only CXCL-10 exhibited significant augmentation. n=5. Data were analyzed by Student's t-test. **P<0.01. IL, interleukin; CCL, C-C motif chemokine ligand; CXCL, C-X-C motif chemokine ligand.

Journal: Experimental and Therapeutic Medicine

Article Title: Senescent hepatocytes enhance natural killer cell activity via the CXCL-10/CXCR3 axis

doi: 10.3892/etm.2019.8037

Figure Lengend Snippet: Cytokines in cell-free culture supernatants of senescent hepatocytes. (A) Representative images of cytokine arrays. 1, CCL-2; 2, CXCL-1; 3, CXCL-10. (B) Bar graph representing the densitometry of the spotted antibody array results. The secretion of IL-1α, IL-1β, IL-6, CCL-2, CXCL-1 and CXCL-2 was differentially increased. (C) ELISA analysis of CXCL-9, −10 and −11. The levels of only CXCL-10 exhibited significant augmentation. n=5. Data were analyzed by Student's t-test. **P<0.01. IL, interleukin; CCL, C-C motif chemokine ligand; CXCL, C-X-C motif chemokine ligand.

Article Snippet: Alternatively, DMEM/F12/10% FBS containing recombinant CXCL-10 (10 ng/ml; R&D Systems, Inc.) was chosen as the positive control.

Techniques: Ab Array, Enzyme-linked Immunosorbent Assay

Migration of NK cells in response to supernatants from senescent AML12 cells. Anti-CXCR3 or anti-CXCL-10 antibodies were used for the blocking assay. The percentage of NK cell migration was calculated by dividing the number of cells that migrated to the lower well by the total number of cells. n=3. Data were analyzed by one-way ANOVA with Tukey's post hoc test. ***P<0.001. NK, natural killer; CXCR, C-X-C motif chemokine receptor; CXCL, C-X-C motif chemokine ligand.

Journal: Experimental and Therapeutic Medicine

Article Title: Senescent hepatocytes enhance natural killer cell activity via the CXCL-10/CXCR3 axis

doi: 10.3892/etm.2019.8037

Figure Lengend Snippet: Migration of NK cells in response to supernatants from senescent AML12 cells. Anti-CXCR3 or anti-CXCL-10 antibodies were used for the blocking assay. The percentage of NK cell migration was calculated by dividing the number of cells that migrated to the lower well by the total number of cells. n=3. Data were analyzed by one-way ANOVA with Tukey's post hoc test. ***P<0.001. NK, natural killer; CXCR, C-X-C motif chemokine receptor; CXCL, C-X-C motif chemokine ligand.

Article Snippet: Alternatively, DMEM/F12/10% FBS containing recombinant CXCL-10 (10 ng/ml; R&D Systems, Inc.) was chosen as the positive control.

Techniques: Migration, Blocking Assay

CCL18 involves in M2-like TAMs induced EMT and stemness in SCCHN in vitro. Tu686 cells cultured with M2 CM or M0 CM in the presence or absence of an anti-CCL18 neutralizing antibody at 15 μg/mL, or an isotype-matched IgG protein. a – d qRT-PCR assays were used to check mRNA expression of EMT markers E-cadherin ( a ), Vimentin ( b ), and EMT transcription factors Snail ( c ) and Slug ( d ). e , f Representative images for tumorsphere formation and the number of tumorspheres. g , h mRNA expression of CSCs markers CD133 ( g ) and CD44 ( h ) was quantified by qPCR. Data are shown as mean ± SD. * P < 0.05, ** P <0.01, *** P < 0.001, **** P < 0.0001

Journal: Cancer Cell International

Article Title: Tumor-associated macrophages derived CCL18 promotes metastasis in squamous cell carcinoma of the head and neck

doi: 10.1186/s12935-018-0620-1

Figure Lengend Snippet: CCL18 involves in M2-like TAMs induced EMT and stemness in SCCHN in vitro. Tu686 cells cultured with M2 CM or M0 CM in the presence or absence of an anti-CCL18 neutralizing antibody at 15 μg/mL, or an isotype-matched IgG protein. a – d qRT-PCR assays were used to check mRNA expression of EMT markers E-cadherin ( a ), Vimentin ( b ), and EMT transcription factors Snail ( c ) and Slug ( d ). e , f Representative images for tumorsphere formation and the number of tumorspheres. g , h mRNA expression of CSCs markers CD133 ( g ) and CD44 ( h ) was quantified by qPCR. Data are shown as mean ± SD. * P < 0.05, ** P <0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Cells were suspended in tumorsphere medium (serum-free DMEM/F-12 medium supplemented with 20 ng/mL human recombinant epidermal growth factor (EGF; Millipore, MA, USA), 20 ng/mL human recombinant basic fibroblast growth factor (bFGF; PeproTech, NJ, USA) and 2% B27), and then subsequently plated in ultra-low attachment 24-well plates (Corning, NY, USA) at a density of no more than 2000 cells/well.

Techniques: In Vitro, Cell Culture, Quantitative RT-PCR, Expressing